Aseptic Techniques Training

Aseptic Techniques Training

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8 AM - 4 PM MST (M-F)

Faculty: Erin Azuse, RN BSN

Successful Completion: Complete entire module, complete the exam with a passing score of 80% or better, and complete the evaluation form.

Estimated Time to Complete Activity: 60 minutes.

CEUs: HIPAA Exams is authorized by IACET to offer 0.1 CEUs for this program. CEU Information


Healthcare-associated infections are a cause of great concern and are estimated to affect 2 million people each year. Those who work in the healthcare field should perform their duties in a manner that minimizes the spread of infectious microorganisms. Having a solid understanding and employing the principles of aseptic technique will help reach this goal.

Course includes a video and audio component with stand-alone exam

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Learning Objectives

  • Describe why asepsis is important in the health care setting
  • List the difference between medical and surgical asepsis
  • Recall the chain of infection
  • Identify methods of decreasing the spread of infection
  • List the steps in routine hand hygiene and a surgical hand scrub
  • Compare the different ways one can correctly apply sterile gowns and gloves
  • Explain the main principles of surgical asepsis

Table of Contents

Aseptic Technique

Table of Contents:

  • Aseptic Technique
  • Legal Notice
  • Purpose of Course
  • Learning Objectives
  • An Introduction to Asepsis
  • Microorganisms that Cause Infections
  • The Chain of Infection
  • Antiseptics, Disinfectants, and Sterilization
  • OSHA’s Universal Precautions and CDC’s Standard Precautions
  • Main Elements of Standard Precautions
  • CDC’s Transmission-Based Precautions
  • Hand Hygiene
  • Routine Hand Hygiene
  • Hand Hygiene Using Alcohol-Based Antiseptic Hand Rub
  • Sterile/Surgical Hand Hygiene
  • Traditional Surgical Hand Scrub
  • Surgical Hand Scrub Using Alcohol-Based Rub
  • Applying and Removing Sterile Gowns and Gloves
  • Purpose of Sterile Gowns and Gloves
  • Open Method for Applying Sterile Gloves
  • Closed Method for Applying Sterile Gown and Gloves
  • Assisted Method for Applying Sterile Gloves
  • Important Considerations for Applying Sterile Gowns and Gloves
  • Removing Sterile Gown and Gloves
  • Surgical Asepsis
  • Principles of Surgical Asepsis
  • End of Course Quiz

Course Content Example 1:

 An Introduction to Asepsis

Microorganisms exist everywhere – in water, on the skin, within the intestinal tract, and on surfaces.

  • Many are harmless, but some microorganisms can cause disease in body tissues and are referred to as pathogens

In an effort to control the spread of infectious diseases, the principle of asepsis is applied within the health care setting

  • This protects both the health care workers and the patient

Asepsis refers to the absence of disease-causing microorganisms

  • There are two categories of asepsis:
  • Medical asepsis is also known as clean technique. It is the process of limiting the growth and spread of pathogens. Examples are hand hygiene or changing a patient’s dirty linens.
  • Surgical asepsis is also called sterile technique. These are the practices that destroy all microorganisms and their spores. This is used for things like invasive procedures and surgery.

Content Example 2:

 Microorganisms that Cause Infections

Four categories of microorganisms cause diseases in humans

  1. Bacteria are most common disease-causing microorganisms
  2. Examples are E. colie, Staphylococcus aureus, and Enterococcus
  3. Viruses are made of nucleic acid and must enter a living cell to reproduce
  4. Examples are rhinovirus, hepatitis, and HIV
  5. Fungi include yeasts and molds
  6. Ringworm is a common example
  7. Parasites live on other living things
  8. Malaria is caused by a parasitic protozoa

Content Example 3:

The Chain of Infection

Six links must be present to cause an infection:

  1. The etiologic agent
  2. The reservoir
  3. The portal of exit
  4. A method of transmission
  5. A portal of entry
  6. A susceptible host

Aseptic technique attempts to break this chain


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